BLAST

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BLAST - Basic Logical Alignment Search Tool

What it does:


“…finds regions of local similarity between sequences.”

“…compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.”

“… can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.”


Main Page Blast main page.jpg



Example Use: You’ve recently cloned a mouse adrenergic receptor, but you want to know if there are any homologous sequences in the mouse genome that might code for other receptor isoforms

Why not BLAST sequence from your cloned receptor…


BLAST results Blast results.jpg



BLAST Link: http://blast.ncbi.nlm.nih.gov/Blast.cgi